Skip to content
Reagents | Liquid | Solid |
---|
Agar | – | 24g |
Agar | 20g | 20g |
Bacterial peptone | 10g | 10g |
DH2O | 950ml | 950ml |
- Autoclave the mixture.
- Prepare for
- For liquid media:
- Add 50 mL of sterile 40% (w/v) glucose and mix.
- For agar plates:
- While stirring the autoclaved mixture on a magnetic stir plate, add 50 mL of sterile 40% glucose per liter of media
- Pour to sterile plates and cool to solidify.
YPD/5-FOA Plate
- Mix 450ml of YPD agar media with the following while stirring at 65°C:
- 25mg of uracil
- 50ml of Dextrose
- 700mg of ura– powder.
- Autoclave before use.